FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28966435
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29550.013.9364512
GTAGAAC45900.012.44207211
GTACTCG64300.011.4095624
GCGTGAC38800.011.1941851
TACTCGA66400.010.6074465
TCAGGTA60400.010.35125357
TAGAACA59400.09.879712
GGTAGAA59400.09.84143810
CTCGAAT28600.09.8358261
AGTACTC78000.09.7805773
GTGACTC47750.09.2843643
AATCGAA61600.09.2787085
GGAAAAT119400.09.0846791
AAAATCC88850.08.7635093
GAATCGA48800.08.7618564
CAGGTAG67350.08.6470738
AATTGAC43350.08.4612225
CTAGCAC53400.08.33873214
AAAAATC81350.08.3265962
GAATTGA68450.08.1205574
Produced by FastQC (version 0.11.5)