FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397047
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31050.013.9154072
GTAGAAC42450.010.38177611
GCGTGAC45650.010.3521661
GTACTCG59050.010.0612464
GTGACTC49850.09.5253453
TACTCGA63550.08.9587395
GGAAAAT119900.08.896351
GAATCGA51800.08.8627434
AGTACTC72350.08.4293533
TCAGGTA57400.08.3458357
AATCGAA61300.08.2964045
CTAGCAC54550.08.28693814
TAGAACA53350.08.21888312
AATTGAC46100.08.0542475
GGTAGAA56750.07.845058410
AAAATCC95600.07.8152633
GCTCTAG63050.07.703036311
GAATTGA70300.07.65086134
GGGAAAT64350.07.58863831
AAATTGA85900.07.54513554
Produced by FastQC (version 0.11.5)