FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30516463
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26900.013.3014942
GCGTGAC38400.010.9004311
CTCTATA87300.09.02891545
GGAAAAT122300.08.9979661
GAATCGA48200.08.82424
GTAGAAC34850.08.70960611
ACTCGTG89950.08.60410836
GCGGAAA102500.08.5405861
GTACTCG49550.08.5383664
CACTCGT87850.08.52688435
CTCGAAT30100.08.448391
AAAATCC91800.08.4329983
GGGAAAT66900.08.2750571
TACTCGA50550.08.1909115
GTGACTC50450.07.94008353
TCACTCG95100.07.923971734
CTAGCAC51000.07.84915514
AGAACGG172400.07.7799223
AAATCCT94350.07.63253164
GCTCTAT104200.07.56387444
Produced by FastQC (version 0.11.5)