FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36443914
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32250.014.583812
GCGTGAC47700.010.6151691
GTAGAAC48800.010.36646611
GGAAAAT153550.09.4823771
GTGACTC54650.08.9768083
GTACTCG71550.08.586284
CTCGAAT35650.08.5219111
GAATCGA57550.08.4852854
TACTCGA73000.08.4460585
AAAATCC118250.08.43059353
TCAGGTA68700.08.1838377
TAGAACA62650.08.00328912
AATCGAA70850.07.971875
GAATTGA89300.07.88760044
CTAGCAC61100.07.83999314
GCTCTAG69300.07.819010711
GGTAGAA66300.07.7997910
AATTGAC58200.07.73273945
AGTACTC85150.07.69066573
TCACACG35800.07.602744615
Produced by FastQC (version 0.11.5)