FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n02_P3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n02_P3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28058511
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23600.012.1101422
GTACTCG54500.010.94245154
GTAGAAC39800.010.39489111
TACTCGA57950.010.368195
AGTACTC66850.09.4932243
GAATCGA41300.09.3722474
GGAAAAT123100.08.7201051
AATCGAA53650.08.598635
GAATTGA75600.08.5730624
GCGTGAC36350.08.4196851
ATCTAAC14350.08.3096096
TCAGGTA55050.08.0892287
TAGAACA52650.07.94357512
GGACTTA36950.07.9176124
GGTAGAA55400.07.91428310
GGGAAAT58750.07.852491
CGCGTAT25200.07.76256810
AAAATCC89650.07.7064453
GACTTAT39700.07.59555245
GGTAGGC28100.07.52351337
Produced by FastQC (version 0.11.5)