FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13001660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4150.029.79614315
CGTCTGA4100.029.06291216
CACGTCT4850.024.5678914
CACACGT5300.023.33013312
ACACGTC5450.021.86300313
AGACCGA3500.020.58491932
CAGTCAC4600.020.05189927
CCGAGAC3650.019.11144829
TCGGAAG8900.017.7073483
CTACGCT1053.137138E-517.12962320
ACCGATC3900.016.73922734
ATCGGAA9600.016.6457672
GAGACCG4450.016.19219431
AGCACAC7600.015.97388610
GCACACG8350.015.34683511
GATCGGA10750.015.2881471
AGCGGTT5300.015.27063311
CCAGTCA6200.015.24007426
CGGAAGA11150.014.9417794
GTCCTAT1301.9155217E-413.85720843
Produced by FastQC (version 0.11.5)