FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28588422
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC35700.025.92713238
ACGTCTG39700.024.64179215
CGTCTGA43600.022.59241516
CCGTACG43500.021.7415233
ACCGTAC51600.018.98150632
CTCGTAT33550.018.0594544
CACCGTA52850.017.84537131
TCGGAAG56950.017.5738893
ATCGGAA57450.017.225232
GTAATCT55300.016.94266739
CACGTCT59800.016.77242314
CACACGT62800.016.2217212
TCGTATG34750.016.00969745
CAGTCAC69800.014.17603727
TAATCTC68000.013.97810540
ACGTAAT67050.013.97163537
TACGTAA68700.013.66827736
AGTCACC79800.013.0195728
ACACGTC85650.012.36664313
AATCTCG68450.011.97767841
Produced by FastQC (version 0.11.5)