FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30478389
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG29050.025.81153933
AGTCACG36350.021.34403428
ACGTCTG38200.020.84077515
GTTTCGG40600.018.85701434
ACGTTTC40750.018.62011732
TTCGGAA40350.018.52758836
CGTCTGA42550.018.39304416
GTCACGT43400.017.93308829
TTTCGGA42400.017.89715835
TATGGGT55300.015.92539143
CACACGT51250.015.62132912
ATCGGAA53050.015.0510492
TCGGAAT49750.014.75600637
TCGGAAG55050.014.7492573
ATATGGG63650.014.04849842
ACACGTC66300.012.85545713
ATGGGTG71350.012.784965544
CACGTCT66300.012.27884714
CACGTTT65000.011.80809731
CAGTCAC74000.011.54818427
Produced by FastQC (version 0.11.5)