FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36104271
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG56350.023.74565728
ACGTCTG58100.023.61181315
CGTCTGA61800.021.97987716
CACGTGG62000.021.16544231
GTCACGT70500.018.88854229
TCGGAAG77250.018.0219613
ATCGGAA78450.017.6029682
CTCGTAT45550.017.40572244
CACGTCT79350.017.28806914
GGCCTTA73750.017.19006336
ACGTGGC77550.017.10101332
GCCTTAT74550.016.9457537
CACACGT82100.016.81846612
TCGTATG45500.015.79111645
TCACGTG86500.015.27071330
TATCTCG68500.014.79646141
CAGTCAC97100.014.31299127
CTTATCT89600.013.5484139
ACACGTC108050.013.445250513
CCTTATC94700.013.1982338
Produced by FastQC (version 0.11.5)