FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29108556
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG33500.018.39357228
ACGTCTG34900.018.3007215
CGTCTGA38700.016.678216
TCGTATG32250.015.92332145
CGTGAAA38650.015.84653633
CTCGTAT34300.014.57784644
TGAAACG43000.014.34841135
GTCACGT45700.013.68387429
AACGATC41100.013.42466638
CACGTGA45850.013.30392731
ACGTGAA46250.013.0953632
ATCGGAA51600.012.5968562
AAACGAT49300.012.37858337
TCGGAAG54100.012.3473143
CACGTCT55400.011.60962214
CACACGT56550.011.45302512
TCACGTG57550.011.02673430
TATAGGC59350.010.57167527
GAAACGA57400.010.47431336
CAGTCAC68300.09.5814827
Produced by FastQC (version 0.11.5)