FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28966435
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG33250.018.87036728
ACGTCTG34950.018.46802515
CGTCTGA38750.016.77307316
ACGTCCG40250.015.27133132
CTCGTAT31000.015.25736544
GTCCGCA39900.015.06775334
TCGTATG33200.013.29646545
CCGCACA48250.013.25372236
GTCACGT48600.013.05248129
TCGGAAG52000.012.9323773
ATCGGAA54050.012.15064052
CACGTCT58800.011.81825614
TATAGGC62500.011.40635827
CACACGT59650.011.1596312
TCCGCAC60400.010.81120735
TCACGTC58600.010.79045330
CAGTCAC64200.010.68396927
ACTATTA69350.010.15041219
TACTATT71500.010.002460518
TTATAGG70000.09.9593626
Produced by FastQC (version 0.11.5)