FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397047
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44950.023.8149915
CGTCTGA45950.023.54146216
GTCCGCA50500.020.02139336
TCGGAAG62450.017.5747183
ATCGGAA62350.017.4226072
CACGTCT64350.017.22914
CTCGTAT32550.016.40003244
CACACGT70100.015.71940812
ACCCGTC71500.014.83227332
TCCGCAT67650.014.58003337
CAGTCAC74100.014.38523227
CGCATCT62800.014.23797239
TCACCCG77050.013.81434730
ACACGTC89850.012.88983313
CCGTCCG90050.012.77819134
TCGTATG37550.012.776587545
CGTCCGC92050.012.52503835
CCCGTCC85850.012.51142333
AGTCACC90950.012.26410128
GTCACCC94300.011.44970229
Produced by FastQC (version 0.11.5)