FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30516463
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49850.022.73735815
CGTCTGA50500.022.3556316
CACGTCT68400.016.9979114
TCGGAAG66500.016.8089263
ATCGGAA67900.016.462352
CACACGT77450.015.30194812
GTCAGAA72200.015.28235636
GTCACAT78950.014.53060729
AGTCACA79000.014.4608628
CAGTCAC79650.014.37108327
TGTCAGA81100.013.8272935
ACACGTC90500.013.26936713
CGGAAGA99500.011.9348244
ATGTCAG102850.011.4505434
CCAGTCA101950.011.2276326
GATCGGA101500.011.1935151
ACATGTC106700.011.05730132
CACATGT112150.010.99856631
AGCACAC111750.010.92722410
GTCGGTT29800.010.8714481
Produced by FastQC (version 0.11.5)