FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36443914
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG35750.023.6837334
ACGTCTG40550.021.46310415
TCCGTAT38500.020.64791137
CGTATCT36850.020.29199839
CGTCTGA45450.019.19865616
CCGTATC40250.019.08022338
TTCCGTA52150.015.3721936
CTCGTAT36950.014.63007844
GTTCCGT58150.014.44434835
TCGGAAG63500.013.8132183
CACGTCT66150.013.76835714
ATCGGAA62250.013.6209992
TCGTATG37450.012.75057345
CACACGT69450.012.62832412
TATAGGC74500.011.59119827
GTCACAG77350.011.45780229
CAGTCAC78150.011.39513727
CACAGTT82100.010.77459531
AGTCACA81200.010.579391528
ATCGGTG127950.010.5111596
Produced by FastQC (version 0.11.5)