FastQCFastQC Report
Sat 4 May 2019
CCLM1ACXX_l01_n01_P3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l01_n01_P3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28058511
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37150.022.94342615
CGTCTGA39300.021.68829216
CTCGTAT32950.017.15767544
CACGTCT52850.016.76535814
ATCGGAA52700.016.1749462
TCGGAAG54050.016.0621173
TCGTATG33950.015.32533545
CACAGTC56250.015.16580531
CACACGT61000.014.56218612
AGTCAAC61250.013.52897234
CAGTCAC68000.012.56704727
GTCACAG66900.012.54161629
ACACGTC75250.012.22295313
TCACAGT69400.012.19152130
TATAGGC60500.012.15488727
AGTCACA71200.011.71797228
ACAGTCA80450.011.1948932
AATCTCG65350.010.92575541
CGGAAGA86600.010.7261364
GATCGGA82900.010.5028081
Produced by FastQC (version 0.11.5)