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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-10, 21:04 based on data in: /beegfs/mk5636/logs/html/CBY82ACXX/8

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CBY82ACXX_l08_n01_S1S1
        67.7%
        52%
        24.9
        CBY82ACXX_l08_n01_S1S2
        68.8%
        52%
        27.1
        CBY82ACXX_l08_n01_S1S3
        66.2%
        51%
        26.8
        CBY82ACXX_l08_n01_S1S4
        69.5%
        53%
        26.4
        CBY82ACXX_l08_n01_S1S5
        67.4%
        53%
        24.9
        CBY82ACXX_l08_n01_S1S6
        68.7%
        52%
        30.5
        CBY82ACXX_l08_n01_S1S7
        60.1%
        50%
        24.9
        CBY82ACXX_l08_n01_S1S8
        60.1%
        51%
        23.0
        CBY82ACXX_l08_n01_S1S9
        66.7%
        52%
        22.9
        CBY82ACXX_l08_n01_undetermined
        90.2%
        45%
        17.2
        CBY82ACXX_l08_n02_S1S1
        68.9%
        54%
        24.9
        CBY82ACXX_l08_n02_S1S2
        70.4%
        54%
        27.1
        CBY82ACXX_l08_n02_S1S3
        67.7%
        53%
        26.8
        CBY82ACXX_l08_n02_S1S4
        70.5%
        55%
        26.4
        CBY82ACXX_l08_n02_S1S5
        68.7%
        55%
        24.9
        CBY82ACXX_l08_n02_S1S6
        69.5%
        54%
        30.5
        CBY82ACXX_l08_n02_S1S7
        62.9%
        52%
        24.9
        CBY82ACXX_l08_n02_S1S8
        62.1%
        52%
        23.0
        CBY82ACXX_l08_n02_S1S9
        68.1%
        54%
        22.9
        CBY82ACXX_l08_n02_undetermined
        89.0%
        45%
        17.2

        Lane 8 Demultiplexing Report

        Lane 8 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        17,181,655
        6.9
        S1S1
        24,938,527
        10.0
        S1S2
        27,092,781
        10.9
        S1S3
        26,840,429
        10.8
        S1S4
        26,385,938
        10.6
        S1S5
        24,944,388
        10.0
        S1S6
        30,527,627
        12.3
        S1S7
        24,945,196
        10.0
        S1S8
        22,957,039
        9.2
        S1S9
        22,943,162
        9.2

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CCCCCC
        99459.0
        0.6
        TAGGCA
        68806.0
        0.4
        CGCCCC
        68620.0
        0.4
        CGGCCC
        67798.0
        0.4
        GCCCAA
        64498.0
        0.4
        CAGCCC
        60457.0
        0.3
        CCGCCC
        58195.0
        0.3
        GATGTA
        57478.0
        0.3
        GCCCCC
        55513.0
        0.3
        CGGCCA
        54730.0
        0.3
        TGCCCC
        54001.0
        0.3
        GCCCCT
        53088.0
        0.3
        CACCCC
        52713.0
        0.3
        CTCCCC
        52179.0
        0.3
        CCCCCA
        50726.0
        0.3
        CCGCTC
        50218.0
        0.3
        CGCCCA
        48433.0
        0.3
        GCCCAC
        47346.0
        0.3
        GCAATA
        45702.0
        0.3
        TCCCCC
        44752.0
        0.3

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        8.0
        262,848,992
        248,756,742
        6.9
        7.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.