FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17181655
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN384350.22369789173394533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8450.020.8691522
TTCCCTA5900.016.0277988
GTGTAGA4100.014.80944230
AGTGTAG3950.014.80249929
TCCCTAC6200.014.5133589
TACACGA4900.014.22794313
GAGTGTA4150.014.08974228
TCTCGGT3950.013.66093337
ATCGGAA12300.013.6017122
GTAGATC4350.013.44103632
TCGGAAG12450.013.4302253
CGGAAGA14150.012.7666824
GATCGGA13350.012.704361
TTTCCCT10100.012.0456357
TAGATCT4750.011.83569533
GTAGAAC3804.9112714E-1111.24473111
GTGTAGG7050.011.16413416
TCGTGTA6650.011.16018414
CCCTACA8150.011.03777310
AGCGGTT7800.010.95640510
Produced by FastQC (version 0.11.5)