FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22943162
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN436020.1900435519742222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29300.010.51622111
GTACTCG52150.09.7409834
CGTGACT23000.09.729562
ACACGCG20800.09.1017686
TACTCGA56150.08.8381365
AGTACTC61350.08.8006253
TCAGGTA40000.08.61627
GCGTGAC30600.08.2014291
GGTAGAA40450.07.78424110
AGAACGG137000.07.6841443
AATCGAA45650.07.65911675
AGTCTCG13550.07.65784035
GAATCGA33150.07.62797164
GGAAAAT93450.07.50015741
CAGGTAG43000.07.43390668
GGACTTA29900.07.3999624
CTCGAAT19600.07.38264271
AATTGAC36950.07.3257915
GAAAAAT114050.07.2952551
TAGAACA42900.07.287228612
Produced by FastQC (version 0.11.5)