FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22957039
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN434260.1891620256427669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC34950.08.42987611
GGAAAAT118650.08.2130341
GGGAAAT71650.07.47870161
AAAATCC85800.07.13555863
GGGGAAT55800.07.1313631
AAAAATC85050.07.0700152
CAGGTAG43200.06.8784848
AAATCGG27350.06.85168034
TCAGGTA49550.06.8189747
CGGGAAT19900.06.8169831
GAATTGA67050.06.80187854
CCGGAAT23050.06.7681851
AATTCAC36800.06.7427855
GGAAGAT165000.06.7143841
AAATCTA40450.06.6420894
AATCGGT23200.06.6117345
AAATCCA90650.06.50053364
AATCGAA47250.06.49279265
CGAAAAT31500.06.45990321
CCCGAAT18200.06.45990321
Produced by FastQC (version 0.11.5)