FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24945196
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47500.09.61183511
TCAGGTA54500.09.0928047
CAGGTAG51300.09.03937158
TAATCGA33950.08.9776074
CTAATCG25050.08.3871193
AATCGAG51600.08.26230055
GGAAAAT124600.07.98395441
TAGAACA59600.07.62267212
GGTAGAA61450.07.53955510
AATCGGA29950.07.45636655
ATCAGGT89400.07.36147176
GGGGAAT59350.07.3141511
CATAAGC87000.07.27543167
AGGTAGA65100.07.25694759
AAAATCC85100.07.14102133
CCCGAAT20750.07.0823711
AAAAATC109300.07.0729522
AATCGGT21700.07.0686755
AAATCGG23450.06.93196344
AATCCAA103150.06.8229825
Produced by FastQC (version 0.11.5)