FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30527627
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT30450.011.5803952
GTAGAAC50700.010.2915611
AATCGAA58400.09.6947435
GCGTGAC40700.09.6105341
TCAGGTA60200.09.4287567
AGCCTAG32250.08.78689825
GAATCGA48800.08.743724
TCGGGGG74850.08.55984439
CTCGAAT27050.08.5256741
GTACTCG55650.08.2354084
GAATTGA77800.08.1541934
CAGGTAG68300.08.1075248
GGAAAAT127100.08.0228161
TAGAACA66450.08.02141512
GGTAGAA67650.07.945670610
AATTGAC51450.07.89155445
GGGGGCC113100.07.88710344
GTGACTC50100.07.7105163
TACTCGA60050.07.70045335
AGAACGG186800.07.61883833
Produced by FastQC (version 0.11.5)