FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24944388
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN470330.1885514288825206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26200.013.9772692
GCGTGAC35450.011.1614551
AGCCTAG27150.010.35454325
GTAGAAC32000.09.69911411
GTACTCG57750.09.4215964
GAATCGA37900.09.41194
GTGACTC41800.09.4044273
AATCGAA42400.08.93763355
AAAATCC74800.08.7590263
TACTCGA61900.08.6729215
GGAAAAT104250.08.5668281
AGTACTC67050.08.5584343
CTCGAAT22300.08.5167411
TAGAAAC34750.08.34839317
GCCTAGA33550.08.31237526
TCAGGTA43850.08.0648367
GCTCTAG53350.07.967651411
AATCGAC26300.07.8048535
GAATTGA61800.07.78127154
AAGCCTA36800.07.70040324
Produced by FastQC (version 0.11.5)