FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26385938
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN502060.19027559300715402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28000.015.3384762
GCGTGAC39500.011.4474391
GTAGAAC36750.011.01583111
GTGACTC46900.010.0194333
GTACTCG64200.09.9875334
TCAGGTA46000.09.4020817
AGTACTC73200.09.228113
GAATCGA37700.09.0429574
GGAAAAT116000.09.0044971
TACTCGA69300.08.9837415
AATCGAA47000.08.878825
TGACTCT55450.08.6319564
CTCGAAT19750.08.4711051
GCTCTAG55350.08.32987311
GGTAGAA49800.08.26463810
TAGAACA51500.08.07912212
AAAATCC75900.08.0664083
ACACGCG25450.07.97027876
GAATTGA65300.07.88309144
ATCTAAC14300.07.88048176
Produced by FastQC (version 0.11.5)