FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26840429
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC46600.010.81095911
TCAGGTA59050.09.6509767
CGTGACT22000.09.4526712
TAGAACA57650.08.77774312
CAGGTAG62400.08.5136558
GCGTGAC31350.08.29351
GGAAAAT143900.08.2170971
GTACTCG51500.08.1538054
GAATCGA44350.07.9920944
AAAATCC92500.07.9129323
GGTAGAA66200.07.88190710
AATCGAA57050.07.630965
GAATTGA76300.07.51560164
AATCGAG47000.07.48693855
AATTGAC49100.07.3504855
AGATCAG110750.07.31821784
AAATCCT98800.07.22079044
AGGTAGA73450.07.1976019
TACTCGA56000.07.129565
TAATCGA28250.07.11256364
Produced by FastQC (version 0.11.5)