FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092781
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26600.012.8315852
GCGTGAC35450.010.2681371
GTAGAAC35100.09.8036511
GTACTCG63600.09.5847764
AATTGAC47300.09.1088315
AGTACTC72400.09.0181133
GAATCGA41500.08.9221824
TACTCGA67250.08.7546475
GTGACTC43550.08.5595743
GGAAAAT124050.08.4567451
TCAGGTA45400.08.1862517
CTCGAAT23500.08.1777351
AATCGAC28900.08.1175685
GGGAAAT60750.08.0387681
GAATTGA73050.08.0357884
GCTCTAG52900.07.737829711
AATCGAA51950.07.6421855
CGAAAAT26200.07.5938741
AGAACGG172200.07.5700543
TCACACG26900.07.52465215
Produced by FastQC (version 0.11.5)