FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n02_S1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n02_S1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24938527
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN475080.19050042530579292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25900.012.6552342
GCGTGAC34750.010.4102921
GTAGAAC32650.09.85033711
GAATTGA63950.09.3206234
GGAAAAT112900.09.0519511
GTGACTC38900.08.6535743
TCAGGTA42350.08.4570987
GTACTCG55650.08.3576394
GAATCGA37950.08.3290964
AAAATCC82050.08.2053383
AATTGAC43350.07.96147975
AATCGAA48650.07.88238335
AGTACTC60900.07.8275163
AAATCCT84200.07.80301574
TACTCGA59750.07.70164875
AGAACGG141250.07.56539253
AAATCCA89050.07.55551244
CTCGAAT19350.07.3613471
AATCCTT73950.07.29039765
GCTCTAG50000.07.24192711
Produced by FastQC (version 0.11.5)