FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17181655
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13000.036.3415115
CGTCTGA13300.035.18347516
CACGTCT14850.032.2686114
CACACGT14800.032.21398512
ACACGTC15100.031.43638413
TCGGAAG18450.029.261153
ATCGGAA19200.028.1173972
CAGTCAC11850.026.98505627
AGCACAC19050.025.38107510
GCACACG19050.025.38107511
CCAGTCA13950.025.18293226
GATCGGA22150.024.3766881
CGGAAGA22250.023.9603714
AACTCCA22700.020.22280522
CTCCAGT20950.019.55964524
CTGAACT27700.017.62477119
GAACTCC28400.016.94959421
AGAGCAC28950.016.6246288
CCCTACA4350.015.51286641
TCCCTAC4150.015.17489640
Produced by FastQC (version 0.11.5)