FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22943162
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC299370.13048332222036352TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG56700.028.88284315
AGTCACG56050.028.35461228
CGTCTGA58400.027.65688316
TCGTATG53400.027.30521643
CTCGTAT54550.026.44065942
CGTATGC60900.023.86886844
TCGGAAG70850.023.4652713
ATCGGAA72000.023.0898252
CACGTCT73700.022.70895214
GTCACGA70500.022.70257629
CACACGT79100.021.21502512
CAGTCAC78400.020.75949927
ACACGTC86600.019.43011313
TCACGAT87850.018.1420430
GATCGGA93550.017.7499431
TCTCGTA82400.017.72097841
CGGAAGA99600.017.1663914
ACGATCA95000.016.98924432
GTATGCC88050.016.91785245
CCAGTCA97850.016.61023326
Produced by FastQC (version 0.11.5)