FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22957039
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC536000.23347958767679056TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG135900.035.24120715
CGTCTGA138500.034.61212516
TCGGAAG150150.032.3778273
ATCGGAA150150.032.2118232
CTCGTAT90300.031.19900742
GTCACAC149000.031.16667429
CACGTCT154950.031.03921514
TCGTATG86900.030.78867543
CACACGT157200.030.69431912
ACACGTC157950.030.64906713
GATCGGA160950.030.3910331
CGTATGC82700.029.79485744
CAGTCAC161350.029.074127
AATCTCG126850.027.8264239
AGTCACA167750.027.75016228
GTCTGAA174300.027.60628917
CACACTT170050.027.53355631
TCACACT173600.026.9706330
CGGAAGA182800.026.7549114
ACACTTG176400.026.61833832
Produced by FastQC (version 0.11.5)