FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24945196
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG65450.021.92832615
CTCGTAT53650.021.18067242
CGTCTGA68250.021.02870216
TCGTATG54400.020.1858843
CGTATGC54700.018.80013344
ATCGGAA79050.018.2419592
CACACGT83000.017.45383612
TCGGAAG83350.017.4094873
ACACGTC86300.017.02146113
CACGTCT85650.016.91425914
GATCGGA93650.015.47209551
TCTCGTA78750.014.94297341
CAGTCAC98150.014.81564327
AGTCACC99200.014.79497528
GTATGCC74450.014.44758245
GTCTGAA107300.013.75303517
CGGAAGA110900.013.7135134
GCACACG123600.012.13918711
GAGCACA127050.012.1112739
CCAGTCA119900.012.09052126
Produced by FastQC (version 0.11.5)