FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30527627
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1840310.6028342786027883TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG278000.038.3474815
CTCGTAT240700.038.33838742
TCGTATG237050.038.02766843
AGTCACG272750.037.95636728
CGTCTGA282150.037.71960416
TCGGAAG295750.036.505053
ATCGGAA297200.036.4548572
TATCTCG271400.036.0910839
CGTATGC242450.036.07663344
GTCACGC288800.035.96386729
CACGTCT301650.035.4901114
CACACGT307050.034.90905412
ACGCCAA295300.034.87375332
CGCCAAT294300.034.8518933
CAGTCAC304800.034.2237127
ACACGTC317850.033.7874313
CACGCCA307050.033.72360631
GATCGGA326100.033.3733021
GTATGCC265150.033.25113745
TCTCGTA280850.033.00699641
Produced by FastQC (version 0.11.5)