FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24944388
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC375400.15049477261177946TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67650.029.40512843
ACGTCTG72000.028.80686415
CGTCTGA71550.028.79939816
CTCGTAT68000.028.75681342
CGTATGC75300.025.8502144
TCGGAAG86600.024.3412443
ATCGGAA89150.023.6950422
CACGTCT90450.023.3536414
CACACGT94400.022.49496712
GTCACAC91300.022.28827929
CAGTCAC92700.021.80597327
ACACGTC100600.021.2880813
AGTCACA97450.020.8585828
AGTGATC95500.020.80009536
TCTCGTA99050.019.99012241
GTATGCC101550.019.72209545
CACAGTG103100.019.58191333
CGGAAGA115050.018.811014
ACACAGT108950.018.55288132
CCAGTCA111650.018.306626
Produced by FastQC (version 0.11.5)