FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26385938
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC435220.164943918234023TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG92300.032.8778615
CGTCTGA94900.031.90598516
CTCGTAT76650.031.1769242
TCGTATG77150.030.77139943
AGTCACT103450.029.00565528
TCGGAAG110150.028.1443313
ATCGGAA110350.027.990592
CACGTCT114900.026.64595814
CGTATGC86050.026.62140544
ACTGACC112500.026.55145332
CACACGT119650.025.90726512
CAGTCAC118600.025.47128727
AATCTCG108850.025.02881639
GTCACTG123300.024.50036229
ACACGTC131250.023.73797813
TCTCGTA104050.023.57020241
CACTGAC130150.023.14164231
GACCAAT133150.022.30129835
GATCGGA139350.022.2977731
TGACCAA134250.022.15548134
Produced by FastQC (version 0.11.5)