FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26840429
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG58350.027.46601732
ACGTCTG61000.026.99466915
CGTCTGA65600.025.37608716
TCGTATG52400.025.03638543
CTCGTAT55650.023.97806542
TCACTTA70400.023.11732530
CTTAGGC70700.023.04853633
TTAGGCA73950.021.7835334
ATCGGAA77700.021.3093262
TCGGAAG79600.021.0277233
CGTATGC60350.020.99295244
AGTCACT78600.020.5910428
CACACGT81200.020.52827512
GTCACTT82050.019.80753929
CACGTCT84500.019.64700714
ACACGTC87300.019.3518513
CACTTAG92100.017.69501131
TAGGCAT93400.017.41355135
CAGTCAC95550.017.24456827
GATCGGA98950.017.0079881
Produced by FastQC (version 0.11.5)