FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092781
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51100.023.59612815
CGTCTGA51850.023.29820316
TCGTATG45850.022.28209943
CTCGTAT49600.020.73334142
TCGGAAG65300.018.9833893
CACGTCT67050.018.38563514
CACCGAT67650.017.90064631
ATCGGAA69100.017.8409752
ACCGATG67450.017.78627632
GATGTAT67550.017.7501835
CGTATGC57750.017.53477144
TATCTCG64950.017.07682439
CACACGT76650.016.25869412
CAGTCAC78100.015.96667827
AGTCACC87300.014.4387528
ACACGTC88200.014.25739313
GTATCTC85700.013.78143938
TCTCGTA79100.013.56842241
GATCGGA93100.013.5097461
TGTATCT92850.012.81710337
Produced by FastQC (version 0.11.5)