FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l08_n01_S1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l08_n01_S1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24938527
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51250.027.78399815
CGTCTGA54100.026.40349216
TCGTATG50550.025.55213243
CTCGTAT50750.024.20946742
CGTATGC58500.021.810444
TCGGAAG68800.021.0253873
ATCGGAA69300.020.938112
CACGTCT69950.020.83877414
CACACGT77750.018.69010712
CAGTCAC76400.018.44470227
GTCACAT81950.017.30542429
AGTCACA82350.017.30337128
ACACGTC85500.017.2855913
TCTCGTA75800.016.71170641
TCACGAT92950.015.82778835
GATCGGA93800.015.6634231
CGGAAGA100850.015.1021444
ATCACGA99900.014.84162534
AGCACAC102350.014.79149810
CCAGTCA98050.014.48679726
Produced by FastQC (version 0.11.5)