FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573487
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310800.1587862193384347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10450.029.6654032
TTCCCTA7650.024.72478
TCCCTAC7500.022.4877059
CCCTACA8200.020.56375510
TTTCCCT10300.019.9047647
TACACGA6750.019.32034313
GTGTAGA5750.018.77436830
GAGTGTA5750.018.77398328
GTAGATC5700.018.5444932
TTTCGGG3400.018.50998937
AGTGTAG5700.018.14992729
TCTCGGT5850.018.05797437
CCTACAC9100.017.78978211
AGCGGTT6150.017.54773710
TAGATCT6100.017.32841533
GGGGGCC10900.016.69410544
ATTTCGG2650.016.11702336
CTCGGTG8150.015.71897738
GTGTAGG6850.015.42730717
TCGGGGG10200.014.98312639
Produced by FastQC (version 0.11.5)