FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19770518
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN301670.15258578455051103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT19750.012.4841772
GTAGAAC26100.010.42526811
GCGTGAC27450.010.2235471
GTACTCG43250.09.9262224
TCGGGGG46550.09.65724739
GGGGGCC64100.09.21826444
GGGGGGC103400.09.08348143
TACTCGA47200.08.9912175
GTGACTC31550.08.5281163
AGTACTC53500.08.4524333
TGGGGGG74850.08.37786540
AATCGAA37250.08.3022725
TTTCGGG20900.08.17408237
GGTAGAA36950.08.15475110
ATTTCGG17400.08.13969436
GGGGCCG51200.08.03029445
AAAGGGG63750.07.972223326
GGAAAAT84800.07.92652461
TTCGGGG50800.07.92041138
ATTCGGG21250.07.82788537
Produced by FastQC (version 0.11.5)