FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20295039
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN309870.15268263342583377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29350.011.18644511
GTACTCG39700.09.6752174
CGTGACT19850.09.229632
AGTACTC44100.09.1765383
TACTCGA43150.08.945375
AATTGAC33100.08.86545
TCAGGTA41700.08.6410217
TAGAACA39100.08.51222412
GGTAGAA40750.08.332540510
CAGGTAG41400.08.3177888
AAAATCC68550.08.245123
GGAAAAT97650.08.226941
CTCGAAT17900.08.091131
GAATTGA56050.08.0622584
GGGGGGC86950.07.67503243
GCGTGAC26000.07.39822961
GGGGGCC61300.07.366939544
ATTGACC34500.07.25008446
AAATCCT74700.07.2290234
TCGGGGG42050.07.21620939
Produced by FastQC (version 0.11.5)