FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19034010
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN292600.15372483254973596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC28900.010.03786611
GAATTGA49900.08.6032124
TCAGGTA38350.08.4558237
GGAAAAT81350.08.4019661
AATCGAA36000.08.1510155
CAGGTAG38450.08.077548
GGTAGAA39750.07.750149710
GAATCGA32700.07.53158434
CTCGAAT18350.07.40025471
GAAAAAT113000.07.35053831
AAAAATC61350.07.30035072
GTACTCG28100.07.23675354
AAAATCC59900.07.1715643
CGAAAAC30100.06.9927491
AATTGAC34350.06.96546275
TAGAACA41600.06.91947312
AGATCAG75000.06.8386784
AGAACGG107650.06.80484253
AATTGAA57550.06.78534035
AGGTAGA48150.06.7727099
Produced by FastQC (version 0.11.5)