FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20956583
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN315240.1504252864123889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGG22300.011.89487637
TCGGGGG64850.010.91815139
GGGGGCC77200.010.767755544
GGGGGGC118600.010.6851343
AGCCTAG21200.010.18320425
TTCGGGG57650.010.137538
ATTTCGG20350.09.72173136
GGGGCCG60550.09.42449845
GGGGGAA103800.09.2069721
TTTCGGG23850.09.14252537
GAATCGA31500.08.8229114
CTCGAAT18250.08.8046491
AAAGGGG82150.08.78720426
GTAGAAC30200.08.6377411
TGGGGGG93450.08.58626340
GGAAAAT84650.08.2078241
GAATTGA52300.08.03578954
GTACTCG38750.07.9885174
GGGGAAA94500.07.94791722
AGAACGG133550.07.8889363
Produced by FastQC (version 0.11.5)