FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21788087
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN334130.1533544454820655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22950.012.8115352
GCGTGAC28800.011.0009071
GTACTCG51400.010.4620584
GTAGAAC31550.010.40625111
TACTCGA55700.09.3613695
AGTACTC58300.09.3069683
CTCGAAT19300.09.145981
TCAGGTA41250.09.0630487
AGCCTAG20150.09.03988225
GTGACTC37150.08.7024753
AATTGAC35050.08.2433655
GCTCTAG41050.08.10754411
GGAAAAT96250.08.0646651
GGTAGAA42750.07.8898910
GAATTGA52250.07.8702474
AATCGAA39500.07.82899575
GAATCGA34100.07.8186344
AAAATCC67150.07.81101563
CAGGTAG44350.07.7646648
GCCTAGA23350.07.70477426
Produced by FastQC (version 0.11.5)