FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19616462
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN296710.1512556137798957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC33000.011.03940811
TCAGGTA41350.010.0216267
TAGAACA43100.08.8700112
CAGGTAG46150.08.4858748
AATCGAA39400.08.4792385
GGTAGAA45900.08.42634710
AAAATCC64450.08.3838713
GAATTGA53550.08.3545714
GTACTCG33000.08.353424
GGAAAAT99050.07.9513351
AGGTAGA49150.07.77903279
AAAAATC66300.07.67621952
AGATCAG81450.07.40693664
GGGGGGC76950.07.329184543
AAATCCT68400.07.23446564
TGGGGGG60900.07.19677240
TTTCGGG21050.07.154946337
CGGAAAT18700.07.14045141
AATTGAC35400.07.0780075
AGAGATC90050.07.0583152
Produced by FastQC (version 0.11.5)