FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11756355
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178670.1519773773418717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT13200.012.8524762
GCGTGAC15550.011.643081
GTAGAAC16450.010.11589711
AATCGAA21200.09.3701985
GTGACTC19500.09.2760873
GTACTCG27300.09.0219314
AAAATCC36200.08.9942173
GAATCGA18350.08.9892274
AGTACTC30450.08.984783
GGAAAAT52800.08.8724741
TCACACG12400.08.70497315
CTCGAAT10050.08.5570841
TCAGGTA21650.08.5296747
TACTCGA29500.08.4938185
ACACGCG10700.08.2136416
AAAAATC31200.08.1201282
GGTAGGC11150.08.0790757
AATTGAC18850.08.0235295
GGGAAAT26850.08.0073281
GCTCTAG23850.07.825792311
Produced by FastQC (version 0.11.5)