FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285536
Sequences flagged as poor quality0
Sequence length51
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG85970.26166202409591616Illumina Single End PCR Primer 1 (100% over 51bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42500.12935484499332833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35830.1090537434379048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAA93950.041.0217554
GACCGGA42950.040.917542
TCGGAGA47650.040.013964
TTCGGAA23550.039.8378263
CGGAAAG110350.039.656245
CCGGAAA40600.039.561764
GGATCGG168350.039.5202181
GATTGGA37550.039.5135082
CGGAAAA60800.038.9755065
ATCGGAG45600.038.9669233
AATCCGG29400.038.69510336
ACCGGAA35100.038.6440433
GAATTCC20300.038.3288434
AATTTCG40800.038.13862635
GTTCGGA36200.038.1129882
ACGGAAA29500.037.983214
TTCGGAG16550.037.97393
ATCCGGG40050.037.66342537
TTGGAAA32100.037.6513674
GGTCGGA39500.037.505582
Produced by FastQC (version 0.11.5)