FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n02_C1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n02_C1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19983743
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN305630.15293931672359878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22250.011.793842
GCGTGAC27750.011.0116231
GTAGAAC25350.010.3786511
GTACTCG47550.09.55227854
TACTCGA50400.09.1375725
AGTACTC52300.09.1223873
GTGACTC32850.09.0170333
GGAAAAT83550.08.804761
AATCGAA37450.08.8010045
AGCCTAG20000.08.77026725
AAAATCC59250.08.7011013
GAATCGA31100.08.428674
TCAGGTA34850.08.4009647
GAATTGA48300.08.2343054
AGAACGG127700.08.1981873
CTCGAAT14950.08.1758551
AATTGAC31800.08.163985
AAGAACG154450.07.60162972
GCCTAGA22800.07.49597326
GCCATGT114550.07.479313417
Produced by FastQC (version 0.11.5)