FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573487
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA29350.040.8486816
ACGTCTG29800.040.15635315
CACGTCT30450.039.44689614
CACACGT30700.039.04521612
ACACGTC31000.038.8195913
AGCACAC32500.037.0920110
CAGTCAC21250.036.558927
CCAGTCA22800.035.95013826
GCACACG34100.035.4817211
CTCCAGT29800.034.52467324
ATCGGAA36900.034.4445842
AACTCCA33550.033.74242422
TCGGAAG37800.033.684073
GATCGGA38600.033.1652831
CGGAAGA38850.032.7748644
AGTCACC13100.032.48753728
AGAGCAC39150.030.8494228
CTGAACT39200.030.75686519
GAACTCC38600.030.59870521
AGTACCG2800.030.55989528
Produced by FastQC (version 0.11.5)