FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19770518
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC617020.312090962917613TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG85450.034.44797543
CTCGTAT87650.033.3998942
AGTCACG91700.033.18588328
ACGTCTG94850.032.63381615
CGTCTGA95650.032.29031816
CGTATGC95100.031.02400644
TCGGAAG108450.028.9836083
ATCGGAA108300.028.9403572
GTCACGA104900.028.88122629
CACACGT110300.028.1442212
CACGTCT111350.027.85869814
CAGTCAC112600.027.30622527
TCTCGTA111050.026.58233841
ACACGTC120650.025.87908413
GTATGCC115850.025.70037745
TCACGAT119600.025.40672330
CCAGTCA125750.024.52265226
GATCGGA128700.024.3919891
ACGATCA126800.024.21056232
CGGAAGA132250.024.006454
Produced by FastQC (version 0.11.5)