FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20295039
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC365630.1801573281036809TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG73200.030.69970315
CGTCTGA74500.030.2545916
CTCGTAT60700.029.32476442
TCGTATG60300.028.70131543
CGTATGC63550.026.95045744
TCGGAAG84650.026.8985313
ATCGGAA85850.026.4176482
GTCACAC87250.024.9464329
CACGTCT92400.024.5397714
AATCTCG80800.024.30804839
CACACGT96150.023.67626412
CAGTCAC95550.023.13296927
ACACGTC101800.022.4947913
GATCGGA102300.022.3924581
TCTCGTA81050.022.29223341
AGTCACA99800.022.03491628
CACACTT100150.021.98038331
TCACACT106000.020.7460830
CGGAAGA113200.020.6921224
GTCTGAA109850.020.6824117
Produced by FastQC (version 0.11.5)