FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l07_n01_C1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l07_n01_C1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19034010
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT37800.020.59815842
TCGTATG37750.020.5683743
ACGTCTG42200.020.30896215
CGTCTGA45850.018.69221716
CGTATGC44850.017.21237644
ATCGGAA55800.015.927042
TCGGAAG57900.015.3108793
CACGTCT59900.014.75845614
CACACGT61750.014.38911812
ACACGTC64800.013.88545613
GTATGCC59200.013.34426145
CAGTCAC66150.013.27025527
TCTCGTA62000.012.77472941
AGTCACC71200.012.70815828
GATCGGA72500.012.41558551
CGGAAGA75100.012.1645474
CCAGTCA77650.011.13114826
TCCAGTC80100.010.98683825
GTCTGAA86050.010.56101517
GCACACG88600.010.30780911
Produced by FastQC (version 0.11.5)